IGEM:Groningen/Notebook/iGEM 2011/2011/06/01

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(Autocreate 2011/06/01 Entry for IGEM:Groningen/Notebook/iGEM_2011)
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==Entry title==
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==01-06-11==
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<br>Do a PCR for PhybB on E.coli DH5alpha suspension:
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<br>Composition:
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<br>Taq 10× buffer: 5μl
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<br>dNTPs: 1μl
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<br>MgCl2: 3μl
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<br>taq 5u/μl: 0.25μl
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<br>Forward primer 10mM: 1μl
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<br>Reverseprimer 10mM: 1μl
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<br>Template: 1μl
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<br>MQ: 38.75μl
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<br>
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<br>Analyse them on a 1% agarosegel
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<br>PCR conditions:
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<br>denaturation: 10min
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<br>Cycle 25×
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<br>denaturtaion: 1min
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<br>annealing: 1min (gradient for 60, 65 and 70 degrees)
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<br>extension: 1min
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<br>Final extension: 10min
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<br>Store infinite
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<br>
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<br> Making cloning strategy with biobrick parts
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<br>

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01-06-11


Do a PCR for PhybB on E.coli DH5alpha suspension:
Composition:
Taq 10× buffer: 5μl
dNTPs: 1μl
MgCl2: 3μl
taq 5u/μl: 0.25μl
Forward primer 10mM: 1μl
Reverseprimer 10mM: 1μl
Template: 1μl
MQ: 38.75μl

Analyse them on a 1% agarosegel
PCR conditions:
denaturation: 10min
Cycle 25×
denaturtaion: 1min
annealing: 1min (gradient for 60, 65 and 70 degrees)
extension: 1min
Final extension: 10min
Store infinite

Making cloning strategy with biobrick parts


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