IGEM:Groningen/Notebook/iGEM 2011/2011/06/03: Difference between revisions
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Joyce Mulder (talk | contribs) (Autocreate 2011/06/03 Entry for IGEM:Groningen/Notebook/iGEM_2011) |
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== | ==03-06-11== | ||
<br> Colony PCR of transformants | |||
<br> Mastermix: | |||
<br> Taq 10× buffer: 116μl | |||
<br> dNTPs 10mM: 23.2μl | |||
<br> MgCl2: 69.6μl | |||
<br> Forward Biobrick primer: 23.2μl | |||
<br> Reverse Biobrick primer: 23.2μl | |||
<br> Taq DNA polymerase: 5.8μl | |||
<br> MQ water: 899μl | |||
<br> | |||
<br> PCR conditions: | |||
<br> Preheated lid: 111°C | |||
<br> Denaturation: 94°C for 10 min. | |||
<br> Cycle 33×: | |||
<br> denaturation: 94°C for 30s. | |||
<br> annealing: 60°C for 30s. | |||
<br> Extension: 72°C for 2,5 min. | |||
<br> Final extension: 72°C for 10 min. | |||
<br> Store infinite at 4°C | |||
<br> | |||
<br> Analyse on a 1% TBE agarose gel. | |||
<br> | |||
<br> Working on Human Practices. | |||
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Revision as of 01:49, 21 September 2011
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03-06-11
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