IGEM:Groningen/Notebook/iGEM 2011/2011/06/06
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| + | <br> Plasmid Prep of the biobricks: DT, cI, LasRLVA, RFP,PBADaraC, RFP DT, PlasB, RBS-GFP-DT | ||
| + | <br> | ||
| + | <br> ND1000- nanodrop measurements: | ||
| + | <br> DT: 53.9 ng/μl | ||
| + | <br> cI: 64.1 ng/μl | ||
| + | <br> LasRLVA: 43.3 ng/μl | ||
| + | <br> RFP: 32.9 ng/μl | ||
| + | <br> PBADaraC: 53.2 ng/μl | ||
| + | <br> RFP DT: 53.3 ng/μl | ||
| + | <br> PlasB: 44.8 ng/μl | ||
| + | <br> RBS-GFP-DT: 52.5 ng/μl | ||
| + | <br> | ||
| + | <br> Samples were checked again with restriction analysis. | ||
| + | <br> Digestion mix: | ||
| + | <br> 14μl FD buffer | ||
| + | <br> 3.5μl EcoRI | ||
| + | <br> 3.5μl PstI | ||
| + | <br> 49μl MQ water | ||
| + | <br> 10μl mix+ 10μl DNA sample | ||
| + | <br> | ||
| + | <br> Analyse on a 1% TBE agarose gel | ||
| + | <br> Samples look alright, DT, cI, LasRLVA, RFP, PBADaraC, RFP DT, PlasB, RBS-GFP-DT were send for sequencing | ||
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