IGEM:Groningen/Notebook/iGEM 2011/2011/06/06: Difference between revisions
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Joyce Mulder (talk | contribs) (Autocreate 2011/06/06 Entry for IGEM:Groningen/Notebook/iGEM_2011) |
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== | ==06-06-11== | ||
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<br> Plasmid Prep of the biobricks: DT, cI, LasRLVA, RFP,PBADaraC, RFP DT, PlasB, RBS-GFP-DT | |||
<br> | |||
<br> ND1000- nanodrop measurements: | |||
<br> DT: 53.9 ng/μl | |||
<br> cI: 64.1 ng/μl | |||
<br> LasRLVA: 43.3 ng/μl | |||
<br> RFP: 32.9 ng/μl | |||
<br> PBADaraC: 53.2 ng/μl | |||
<br> RFP DT: 53.3 ng/μl | |||
<br> PlasB: 44.8 ng/μl | |||
<br> RBS-GFP-DT: 52.5 ng/μl | |||
<br> | |||
<br> Samples were checked again with restriction analysis. | |||
<br> Digestion mix: | |||
<br> 14μl FD buffer | |||
<br> 3.5μl EcoRI | |||
<br> 3.5μl PstI | |||
<br> 49μl MQ water | |||
<br> 10μl mix+ 10μl DNA sample | |||
<br> | |||
<br> Analyse on a 1% TBE agarose gel | |||
<br> Samples look alright, DT, cI, LasRLVA, RFP, PBADaraC, RFP DT, PlasB, RBS-GFP-DT were send for sequencing | |||
<br> | |||
Revision as of 01:58, 21 September 2011
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