IGEM:Groningen/Notebook/iGEM 2011/2011/06/06

From OpenWetWare

< IGEM:Groningen | Notebook/iGEM 2011 | 2011 | 06(Difference between revisions)
Jump to: navigation, search
(Autocreate 2011/06/06 Entry for IGEM:Groningen/Notebook/iGEM_2011)
Current revision (03:58, 21 September 2011) (view source)
(Entry title)
 
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
==Entry title==
+
==06-06-11==
-
* Insert content here...
+
 
 +
<br>
 +
<br> Plasmid Prep of the biobricks: DT, cI, LasRLVA, RFP,PBADaraC, RFP DT, PlasB, RBS-GFP-DT
 +
<br>
 +
<br> ND1000- nanodrop measurements:
 +
<br> DT: 53.9 ng/μl
 +
<br> cI: 64.1 ng/μl
 +
<br> LasRLVA: 43.3 ng/μl
 +
<br> RFP: 32.9 ng/μl
 +
<br> PBADaraC: 53.2 ng/μl
 +
<br> RFP DT: 53.3 ng/μl
 +
<br> PlasB: 44.8 ng/μl
 +
<br> RBS-GFP-DT: 52.5 ng/μl
 +
<br>
 +
<br> Samples were checked again with restriction analysis.
 +
<br> Digestion mix:
 +
<br> 14μl FD buffer
 +
<br> 3.5μl EcoRI
 +
<br> 3.5μl PstI
 +
<br> 49μl MQ water
 +
<br> 10μl mix+ 10μl DNA sample
 +
<br>
 +
<br> Analyse on a 1% TBE agarose gel
 +
<br> Samples look alright, DT, cI, LasRLVA, RFP, PBADaraC, RFP DT, PlasB, RBS-GFP-DT were send for sequencing
 +
<br>
 +
 
 +
 
 +
 

Current revision

Project name Main project page
Previous entry      Next entry

06-06-11



Plasmid Prep of the biobricks: DT, cI, LasRLVA, RFP,PBADaraC, RFP DT, PlasB, RBS-GFP-DT

ND1000- nanodrop measurements:
DT: 53.9 ng/μl
cI: 64.1 ng/μl
LasRLVA: 43.3 ng/μl
RFP: 32.9 ng/μl
PBADaraC: 53.2 ng/μl
RFP DT: 53.3 ng/μl
PlasB: 44.8 ng/μl
RBS-GFP-DT: 52.5 ng/μl

Samples were checked again with restriction analysis.
Digestion mix:
14μl FD buffer
3.5μl EcoRI
3.5μl PstI
49μl MQ water
10μl mix+ 10μl DNA sample

Analyse on a 1% TBE agarose gel
Samples look alright, DT, cI, LasRLVA, RFP, PBADaraC, RFP DT, PlasB, RBS-GFP-DT were send for sequencing



Personal tools