Preheated lid: 111°C
Denaturation: 94°C for 10 min.
denaturation: 94°C for 30s.
annealing: 60°C for 30s.
Extension: 72°C for 2,5 min.
Final extension: 72°C for 10 min.
Store infinite at 4°C
Analyse on a 1% TBE agarose gel.
Asked info in team about why some PCR's fail: next time use for taq buffer not the buffer with KCI, but NH4SO4