IGEM:Groningen/Notebook/iGEM 2011/2011/06/13: Difference between revisions
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== | ==13-6-11== | ||
<br> | |||
<br> Plasmid prep of the overnight cultures | |||
<br> ND1000-nanodrop measurements: DNA concentrations are between 30 and 60 ng/μl | |||
<br> | |||
<br> Inoculating 2 erlenmeyers of 200ML , 1 for making competentcells DH5alpha and other one for TOP10 cells | |||
<br> Also TOP10cells will be send to the iGEM Wageningen team. | |||
<br> Also preheat other 2 200ml erlenmeyers | |||
<br> | |||
<br> Restriction analysis: | |||
<br> Digestion test: | |||
<br> 2μl FD buffer | |||
<br> 0.5μl EcoRI | |||
<br> 0.5μl SpeI | |||
<br> 8μl MQ water | |||
<br> 10μl + 10μl sample | |||
<br> | |||
<br> Digestion mix: | |||
<br> 30μl FD buffer | |||
<br> 7.5μl EcoRI | |||
<br> 7.5μl PstI | |||
<br> 120μl MW water | |||
<br> | |||
<br> Digestion mix: | |||
<br> 30μl FD buffer | |||
<br> 7.5μl XbaI | |||
<br> 7.5μl SpeI | |||
<br> 120μl MW water | |||
<br> | |||
<br> Work in Clone manager: check primers PhybB again | |||
<br> | |||
Revision as of 02:32, 21 September 2011
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13-6-11
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