IGEM:Groningen/Notebook/iGEM 2011/2011/06/13

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(Autocreate 2011/06/13 Entry for IGEM:Groningen/Notebook/iGEM_2011)
Current revision (04:32, 21 September 2011) (view source)
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==Entry title==
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==13-6-11==
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* Insert content here...
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<br>
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<br> Plasmid prep of the overnight cultures
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<br> ND1000-nanodrop measurements: DNA concentrations are between 30 and 60 ng/μl
 +
<br>
 +
<br> Inoculating 2 erlenmeyers of 200ML , 1 for making competentcells DH5alpha and other one for TOP10 cells
 +
<br> Also TOP10cells will be send to the iGEM Wageningen team.
 +
<br> Also preheat other 2 200ml erlenmeyers
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<br>
 +
<br> Restriction analysis:
 +
<br> Digestion test:
 +
<br> 2μl FD buffer
 +
<br> 0.5μl EcoRI
 +
<br> 0.5μl SpeI
 +
<br> 8μl MQ water
 +
<br> 10μl + 10μl sample
 +
<br>
 +
<br> Digestion mix:
 +
<br> 30μl FD buffer
 +
<br> 7.5μl EcoRI
 +
<br> 7.5μl PstI
 +
<br> 120μl MW water
 +
<br>
 +
<br> Digestion mix:
 +
<br> 30μl FD buffer
 +
<br> 7.5μl XbaI
 +
<br> 7.5μl SpeI
 +
<br> 120μl MW water
 +
<br>
 +
<br> Work in Clone manager: check primers PhybB again
 +
<br>
 +
 

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13-6-11



Plasmid prep of the overnight cultures
ND1000-nanodrop measurements: DNA concentrations are between 30 and 60 ng/μl

Inoculating 2 erlenmeyers of 200ML , 1 for making competentcells DH5alpha and other one for TOP10 cells
Also TOP10cells will be send to the iGEM Wageningen team.
Also preheat other 2 200ml erlenmeyers

Restriction analysis:
Digestion test:
2μl FD buffer
0.5μl EcoRI
0.5μl SpeI
8μl MQ water
10μl + 10μl sample

Digestion mix:
30μl FD buffer
7.5μl EcoRI
7.5μl PstI
120μl MW water

Digestion mix:
30μl FD buffer
7.5μl XbaI
7.5μl SpeI
120μl MW water

Work in Clone manager: check primers PhybB again


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