IGEM:Groningen/Notebook/iGEM 2011/2011/06/20: Difference between revisions
Joyce Mulder (talk | contribs) (Autocreate 2011/06/20 Entry for IGEM:Groningen/Notebook/iGEM_2011) |
Joyce Mulder (talk | contribs) |
||
| Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==20-6-11== | ||
<br> | |||
<br> PCRs with different samples, scheme: | |||
<br> Samples PhybB: DNA TG1 3×TG1 Colony DH5alpha 3× colonies DH5alpha | |||
<br> pfu 10× buffer: 5μl 15μl 5μl 15μl | |||
<br> dNTPs 10mM: 1μl 3μl 1μl 3μl | |||
<br> Forward Biobrick primer: 1μl 3μl 1μl 3μl | |||
<br> Reverse Biobrick primer: 1μl 3μl 1μl 3μl | |||
<br> Pfu DNA polymerase: 1μl 3μl 1μl 3μl | |||
<br> MQ water: 41μl 123μl 41μl 123μl | |||
<br> | |||
<br> PBAD and PlasI PCRs: | |||
<br> pfu 10× buffer: 5μl | |||
<br> dNTPs 10mM: 1μl | |||
<br> Forward Biobrick primer: 1μl | |||
<br> Reverse Biobrick primer: 1μl | |||
<br> Pfu DNA polymerase: 1μl | |||
<br> MQ water: 41μl | |||
<br> | |||
<br>PCR conditions: | |||
<br>Preheated lid: 111°C | |||
<br>Denaturation: 95°C for 3 min. | |||
<br>Cycle 33×: | |||
<br>denaturation: 95°C for 30s. | |||
<br>annealing: 60°C for 30s. | |||
<br>Extension: 72°C for 2.5 min. | |||
<br>Final extension: 72°C for 10 min. | |||
<br>Store infinite at 4°C | |||
<br> | |||
<br> Analyse samples on a 1% agarose gel | |||
<br> Clean up the DNA samples | |||
<br> work on human practices | |||
Revision as of 02:59, 21 September 2011
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
20-6-11
| |
