IGEM:Groningen/Notebook/iGEM 2011/2011/06/21: Difference between revisions
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Joyce Mulder (talk | contribs) (Autocreate 2011/06/21 Entry for IGEM:Groningen/Notebook/iGEM_2011) |
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== | ==21-6-11== | ||
<br> | |||
<br> Digestion+ ligation PhybB product with taq polymerase | |||
<br> | |||
<br> Purify the PCR product with the High Pure PCR Purification kit from Roche | |||
<br> | |||
<br> Digestion mix: | |||
<br> 18μl buffer | |||
<br> 4.5μl EcoRI | |||
<br> 4.5μl SpeI | |||
<br> 49μl MQ water | |||
<br> | |||
<br> Vector mix: | |||
<br> 18μl buffer | |||
<br> 4.5μl EcoRI | |||
<br> 4.5μl XbaI | |||
<br> 49μl MQ water | |||
<br> | |||
<br> Ligation: | |||
<br> 2μl T4 DNA ligase buffer | |||
<br> 1μl T4 DNA ligase | |||
<br> 7μl insert | |||
<br> 3μl vector | |||
<br> 7μl MQ water | |||
<br> | |||
<br> Ligate 2 hours at room temperature | |||
<br> Ligate further overnight | |||
<br> | |||
<br> Helping with sponsoring | |||
<br> Making researchplan | |||
<br> | |||
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Revision as of 03:25, 21 September 2011
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21-6-11
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