IGEM:Groningen/Notebook/iGEM 2011/2011/06/21

From OpenWetWare

< IGEM:Groningen | Notebook/iGEM 2011 | 2011 | 06
Revision as of 06:25, 21 September 2011 by Joyce Mulder (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search
Project name Main project page
Previous entry      Next entry


Digestion+ ligation PhybB product with taq polymerase

Purify the PCR product with the High Pure PCR Purification kit from Roche

Digestion mix:
18μl buffer
4.5μl EcoRI
4.5μl SpeI
49μl MQ water

Vector mix:
18μl buffer
4.5μl EcoRI
4.5μl XbaI
49μl MQ water

2μl T4 DNA ligase buffer
1μl T4 DNA ligase
7μl insert
3μl vector
7μl MQ water

Ligate 2 hours at room temperature
Ligate further overnight

Helping with sponsoring
Making researchplan

Personal tools