IGEM:Groningen/Notebook/iGEM 2011/2011/07/06: Difference between revisions

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==Entry title==
==6th of July==
* Insert content here...
<br>* Colony PCR of 6 constructs!
<br>HybB promotor in pSB1CR
<br>HybB promotor in RBS GFP ampicillin resistance vector
<br>PcI promotor in RBS GFP ampicillin resistance vector
<br>PlasI promotor in RBS GFP ampicillin resistance vector
<br>LasI-LVA in ampicillin resistance with double terminator vector
<br>cI-LVA in ampicillin resistance with double terminator vector
<br>
<br>Colony PCR protocol:
<br>[[Media: Taq PCR mastermix]]
<br>Preheated Lid: 111° C
<br>Denaturation: 95 ° C for ten minutes
<br>Cycle 33×
<br> Denaturation: 95° C for 30 seconds
<br> Annealing: 60° C for 30 seconds
<br> Extension: 72° C for 1 minute
<br>Final extension: 72° C for ten minutes
<br>Store at 4 ° C (infinite)


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[[category:OWWLabNotebookV1]]
[[category:OWWLabNotebookV1]]

Revision as of 06:40, 8 July 2011

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6th of July


* Colony PCR of 6 constructs!
HybB promotor in pSB1CR
HybB promotor in RBS GFP ampicillin resistance vector
PcI promotor in RBS GFP ampicillin resistance vector
PlasI promotor in RBS GFP ampicillin resistance vector
LasI-LVA in ampicillin resistance with double terminator vector
cI-LVA in ampicillin resistance with double terminator vector

Colony PCR protocol:
Media: Taq PCR mastermix
Preheated Lid: 111° C
Denaturation: 95 ° C for ten minutes
Cycle 33×
Denaturation: 95° C for 30 seconds
Annealing: 60° C for 30 seconds
Extension: 72° C for 1 minute
Final extension: 72° C for ten minutes
Store at 4 ° C (infinite)

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