IGEM:Groningen/Notebook/iGEM 2011/2011/07/08: Difference between revisions
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<br>Check them on agarose gel 1% (TBE). | <br>Check them on agarose gel 1% (TBE). | ||
<br> | <br> No right transformants for cI-LVA and LasR-LVA. | ||
<br> | <br> For next time calculations: | ||
<br> Digestion. cI-LVA: use 3μl for digestion with EcoRI and SpeI and LasR-LVA: use 2μl for digestion | |||
<br> Ligationm. cI-LVA: use 7.8 of purified sample for ligation and LasR-LVA: use 6.5μl of purified sample for ligation. | |||
<br> Digestion of the vector should include FastAP, since so far, there have been only self ligation products. | |||
<br> PCR of pBAD: A lot of unspecific binding products. Another PCR was done with an annealing temperature of 67 and 70 degrees. | |||
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Revision as of 06:00, 8 July 2011
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