IGEM:Groningen/Notebook/iGEM 2011/2011/07/11: Difference between revisions
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<br> - Add to 40μl competent cells 10μl DNA ligation mixture | <br> - Add to 40μl competent cells 10μl DNA ligation mixture | ||
<br> - Incubate 30 minutes on ice | <br> - Incubate 30 minutes on ice | ||
<br> - Do the heatshock: incubate | <br> - Do the heatshock: incubate 1 min at 42°C | ||
<br> - Place the tubes on ice for 2 minutes and then pipette 1 ml of LB medium + 25mM glucose | <br> - Place the tubes on ice for 2 minutes and then pipette 1 ml of LB medium + 25mM glucose | ||
<br> - Incubate the tubes for 1 hour (with maximum of 1.5h) at 37°C | <br> - Incubate the tubes for 1 hour (with maximum of 1.5h) at 37°C |
Revision as of 02:45, 20 September 2011
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11-7-11
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