IGEM:Groningen/Notebook/iGEM 2011/2011/07/25: Difference between revisions
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<br> Digestion vector (with LasR-LVA-DT): | <br> Digestion vector (with LasR-LVA-DT): | ||
<br> | <br> | ||
<br> | <br> 3μl vector | ||
<br> 1μl EcoRI | <br> 1μl EcoRI | ||
<br> 1μl XbaI | <br> 1μl XbaI | ||
<br> 3μl Fast digest buffer | <br> 3μl Fast digest buffer | ||
<br> 1μl Fast AP | <br> 1μl Fast AP | ||
<br> | <br> 21μl MQ water | ||
<br> | <br> | ||
<br> Ligation: | <br> Ligation: |
Revision as of 03:48, 25 July 2011
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