PBAD sample seems to be wrong. Received new one from iGEM Groningen 2009 team.
10× Pfu buffer MgSO4: 5μl
dNTPs 10mM: 1μl
Forward primer 36F: 1μl
Reverse primer 37R: 1μl
(primers contain prefix and suffix)
pfu polymerase: 1μl
Checking PCR products on 1% agarosegel
PCR clean up with kit and measure DNA concentration with ND1000 nanodrop.