IGEM:Groningen/Notebook/iGEM 2011/2011/08/18: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 28: Line 28:
<br> Store infinite at 4°C
<br> Store infinite at 4°C
<br> Analyse PCR samples on a 1% agarosegel
<br> Analyse PCR samples on a 1% agarosegel
<br> PBAD-RBS-GFP-DT (with the new isolated vector) can possibly contain two right clones, colony 7 and 8 since the size is around <br> 2000bp. Colonies 7 and 8 were grown on LB overnight.
<br> Some of the PhybB-taRNA-DT 1 and 4 samples have the right fragment size. Colonies 1.1 and 4.1 were grown on LB overnight.
<br> PhybB-RBS-GFP-DT in TOP 10 cells: all samples have the right fragment size. Colonies 2 and 3 were grown on LB overnight.
<br>
<br>
<br>
<br> Dissolve new ordered primer Forward primer PBAD-araC 623bp
<br> Dissolve new ordered primer Forward primer PBAD-araC 623bp
<br> Prepare some samples for sequencing with this primer:
<br> Prepare some samples for sequencing with this primer:

Revision as of 06:53, 18 August 2011

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

18-8-11


colony PCR of PBAD-RBS-GFP-DT old and new, PhybB-taRNA-DT 1 and 4 and PhybB-RBS-GFP-DT in TOP10 cells:
Composition master mix:
10× Taq buffer: 84μl
dNTPs 10mM: 16.8μl
MgCl2: 50.4μl
Forward biobrickvector primer: 16.8μl
Reverse biobrickvector primer: 16.8μl
Taq polymerase: 4.2μl
MQ water: 651μl

PCR conditions:
Pre heated lid: 111°C
Denaturation: 94°C for 10min
Cycle (33×)
Denaturation: 94°C for 30s
Annealing: 60°C for 30s
Extenstion: 72°C for 2.5min
Final extension: 72°C for 10min
Store infinite at 4°C
Analyse PCR samples on a 1% agarosegel
PBAD-RBS-GFP-DT (with the new isolated vector) can possibly contain two right clones, colony 7 and 8 since the size is around
2000bp. Colonies 7 and 8 were grown on LB overnight.
Some of the PhybB-taRNA-DT 1 and 4 samples have the right fragment size. Colonies 1.1 and 4.1 were grown on LB overnight.
PhybB-RBS-GFP-DT in TOP 10 cells: all samples have the right fragment size. Colonies 2 and 3 were grown on LB overnight.


Dissolve new ordered primer Forward primer PBAD-araC 623bp
Prepare some samples for sequencing with this primer:
PBADaraC-cI-LVA-DT and PBADaraC-LasR-LVA-DT
Filling in forms for stage
Meeting at 14:00 and update meeting at 16:00


Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Groningen/Notebook/iGEM_2011/2011/08/18&oldid=530096"