IGEM:Groningen/Notebook/iGEM 2011/2011/08/24: Difference between revisions
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<br> Store infinite at 4°C | <br> Store infinite at 4°C | ||
<br> Analyse PCR samples on a 1% agarosegel | <br> Analyse PCR samples on a 1% agarosegel | ||
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<br> Transformation of overnight ligation of PBAD-RBS-cI-LVA and PBAD-RBS-LasR-LVA | |||
<br> Add to 40μl competent cells 10μl ligation mixture | |||
<br> Incubate for 30 min on ice | |||
<br> Heat shock: 45s at 42 degrees | |||
<br> Incubate cells on ice for 2 min | |||
<br> Add 1ml LB medium+ 25mM glucose | |||
<br> Incubate cells for 1h/1.5h at 37 degrees | |||
<br> Spin the cells down, resuspend them in 100μl LB medium and plate 90μl and 10μl out on the plates | |||
<br> Put the plates in the stove at 37 degrees overnight | |||
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<br> Made overnight cultures of PhybB-taRNA and PBAD-RBS-GFP for new samples to send for sequencing, since the sequencing results | |||
<br> themselves are shitty, low Q16 and Q20 values and the plot looks shitty. In one peak there is sometimes another smaller peak, | |||
<br> So the primersets and/or the samples could be contaminated | |||
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Revision as of 06:40, 24 August 2011
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24-8-11
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