Pre heated lid: 111°C
Denaturation: 94°C for 10min
Denaturation: 94°C for 30s
Annealing: 60°C for 30s
Extenstion: 72°C for 2min
Final extension: 72°C for 10min
Store infinite at 4°C
Analyse PCR samples on a 1% agarosegel
7 out of 9 for PhybB-RBS-cI-LVA-DT have the correct size and 5 out of 9 for PhybB-RBS-LasR-LVA-DT have the correct size!
Send them monday for sequencing
Plasmid prep of PBAD-RBS-GFP again.
ND-1000 Nanodrop measurements: plot looked better than before.
50 ng/microliter DNA was obtained.
Samples were prepared and send out for sequencing.