# IGEM:Hong Kong HKUST/June 13

### From OpenWetWare

(→Transformer) |
(→Read-out System) |
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===Read-out System=== | ===Read-out System=== | ||

- | for '''Randomizer''' | + | for '''Randomizer Project''' |

by Jean | by Jean | ||

+ | |||

+ | We can find a heterodimer that is exogeneous and can be expressed in ''E.coli''. Applied to Guangnan's model of generating randomness by overlapping promoters, we can put monomer A and monomer B on each side of that promoter to represent outcome signal 0 and 1. Then in the second position, we put them reversely. | ||

+ | |||

+ | '''First Position ''' ----Monomer A (as 0)----promoter----Monomer B (as 1)---- | ||

+ | |||

+ | '''Second Position''' ----Monomer A (as 1)----promoter----Monomer B (as 0)---- | ||

+ | |||

+ | Then only when we get 0 and 0 or 1 and 1 in both positions, we can have both Monomer A and Monomer B, which can be designed to give a signal. | ||

===Summary & Super Brainstorming=== | ===Summary & Super Brainstorming=== |

## Revision as of 03:19, 14 June 2008

## Contents |

## General Info

From 6p.m. to 9p.m.

Rm 5486

Attendance: Xiuye, Jingjing, Cotton, Qing, Jiahui, Carmen, Wisely, Jinyu, Guangnan, Winnie.

## Important Announcement

### Welcome Winnie to our group

### Meeting Schedule for this month

Everyday?! Possibly...

At least,

- June 23, First Draft
- June 30, Second Draft

## Contents

### Transformer

by Qing

Original Idea: Can we make a bacterium that when put into other bacteria, it can change their properties to its own property?

To be simple, the property can be "plamid". In further step, we may change their genomic infomation.

### Tutorial 3

by Wisely

Binomial Distribution, Gaussion Distribution, Markov Process

### Read-out System

for **Randomizer Project**

by Jean

We can find a heterodimer that is exogeneous and can be expressed in *E.coli*. Applied to Guangnan's model of generating randomness by overlapping promoters, we can put monomer A and monomer B on each side of that promoter to represent outcome signal 0 and 1. Then in the second position, we put them reversely.

First Position----Monomer A (as 0)----promoter----Monomer B (as 1)----

Second Position----Monomer A (as 1)----promoter----Monomer B (as 0)----

Then only when we get 0 and 0 or 1 and 1 in both positions, we can have both Monomer A and Monomer B, which can be designed to give a signal.

### Summary & Super Brainstorming

by **Idea Queen** --- Xiuye

- Oscillation
- Polarity
- Transformation competence
- λ phage
- RBS

### Stripe Pattern

by Jingjing

Stripes from Developmental Biology, adapted from Drosophila

3 patterns:

- CDE -- 3 areas (firstly by gradient of A to B)
- hair distributed evenly?
- concentric circles (nutrition)