IGEM:Harvard/2006/Adaptamers/Notebook/2006-8-25

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Repeated bead assay with A20, A35, A50, S20:
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Repeated bead assay with A20, A35, A50, S20 in order to verify Western blot results for A20, A35:
General setup = 40 pmol adaptamer + 200 uL streptavidin beads + 160 pmol thrombin + usual antibodies
General setup = 40 pmol adaptamer + 200 uL streptavidin beads + 160 pmol thrombin + usual antibodies

Revision as of 20:48, 29 October 2006

Repeated bead assay with A20, A35, A50, S20 in order to verify Western blot results for A20, A35:

General setup = 40 pmol adaptamer + 200 uL streptavidin beads + 160 pmol thrombin + usual antibodies

Wells:

A3: Adaptamer = A20 A5: Adaptamer = A35 A7: Adaptamer = A50 A9: Negative control: adaptamer replaced with S20

Image:CstBeadAssay825.jpg

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