IGEM:Harvard/2006/Adaptamers/Notebook/2006-8-25: Difference between revisions
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Wells: | Wells: | ||
A3: Adaptamer = A20 | A3: Adaptamer = A20. Intensity = 3789.42 | ||
A5: Adaptamer = A35 | |||
A7: Adaptamer = A50 | A5: Adaptamer = A35 Intensity = 3030.94 | ||
A9: Negative control: adaptamer replaced with S20 | |||
A7: Adaptamer = A50 Intensity = 1794.28 | |||
A9: Negative control: adaptamer replaced with S20. Intensity = 1810.97 | |||
[[Image:CstBeadAssay825.jpg]] | [[Image:CstBeadAssay825.jpg]] | ||
A20 and A35 were confirmed to exhibit thrombin-targeting function. Adaptamer A50 performed no better than control setup including S20. S20 appeared to have nonspecific thrombin-binding ability. | |||
Latest revision as of 17:50, 29 October 2006
Repeated bead assay with A20, A35, A50, S20 in order to verify Western blot results for A20, A35:
General setup = 40 pmol adaptamer + 200 uL streptavidin beads + 160 pmol thrombin + usual antibodies
Wells:
A3: Adaptamer = A20. Intensity = 3789.42
A5: Adaptamer = A35 Intensity = 3030.94
A7: Adaptamer = A50 Intensity = 1794.28
A9: Negative control: adaptamer replaced with S20. Intensity = 1810.97
A20 and A35 were confirmed to exhibit thrombin-targeting function. Adaptamer A50 performed no better than control setup including S20. S20 appeared to have nonspecific thrombin-binding ability.
