IGEM:Harvard/2006/Adaptamers/Notebook/2006-8-25

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Current revision (20:50, 29 October 2006) (view source)
 
Line 5: Line 5:
Wells:
Wells:
-
A3: Adaptamer = A20
+
A3: Adaptamer = A20. Intensity = 3789.42
-
A5: Adaptamer = A35
+
 
-
A7: Adaptamer = A50
+
A5: Adaptamer = A35 Intensity = 3030.94
-
A9: Negative control: adaptamer replaced with S20
+
 
 +
A7: Adaptamer = A50 Intensity = 1794.28
 +
 
 +
A9: Negative control: adaptamer replaced with S20. Intensity = 1810.97
[[Image:CstBeadAssay825.jpg]]
[[Image:CstBeadAssay825.jpg]]
 +
 +
A20 and A35 were confirmed to exhibit thrombin-targeting function. Adaptamer A50 performed no better than control setup including S20. S20 appeared to have nonspecific thrombin-binding ability.

Current revision

Repeated bead assay with A20, A35, A50, S20 in order to verify Western blot results for A20, A35:

General setup = 40 pmol adaptamer + 200 uL streptavidin beads + 160 pmol thrombin + usual antibodies

Wells:

A3: Adaptamer = A20. Intensity = 3789.42

A5: Adaptamer = A35 Intensity = 3030.94

A7: Adaptamer = A50 Intensity = 1794.28

A9: Negative control: adaptamer replaced with S20. Intensity = 1810.97

Image:CstBeadAssay825.jpg

A20 and A35 were confirmed to exhibit thrombin-targeting function. Adaptamer A50 performed no better than control setup including S20. S20 appeared to have nonspecific thrombin-binding ability.

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