IGEM:Harvard/2006/Adaptamers/Notebook/2006-8-28

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Revision as of 15:37, 29 August 2006

8/28

Strand Displacement round 2

General setup = 40 pmol A20 + 100 uL Streptavidin magnetic beads + 160 pmol thrombin


Well Condition
1general setup
2general setup
3adaptamer = A50
410 pmol R20.20 added
520 pmol R20.20 added
640 pmol R20.20 added
710 pmol R20.35 added
820 pmol R20.35 added
940 pmol R20.35 added
1040 pmol T20 instead of A20
1140 pmol S20 instead of A20
12no thrombin added
  • After 3 washes

Image taken at intensity 3

Image:cst828-displace3washes.jpg

Image:cst828-displace3washesgraph.jpg

  • After 5 washes

Image:cst828-displace5washes.jpg

Image:cst828-displace5washesgraph.jpg

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