IGEM:Harvard/2006/Brainstorming Papers - Jeffrey: Difference between revisions
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**GFP is repressed at low and high concentrations of AHL, and expressed at medium concentrations | **GFP is repressed at low and high concentrations of AHL, and expressed at medium concentrations | ||
**Basu ''et al.'' created three variants with different upper boundaries | **Basu ''et al.'' created three variants with different upper boundaries | ||
***Concentration varies with distance; thus, a mixture of different variants of bacteria produces a bullseye pattern when exposed to AHL | ***Concentration varies with distance; thus, a mixture of different variants of bacteria produces a bullseye pattern when exposed to a point source of AHL | ||
***Multiple AHL sources produce pretty pictures | ***Multiple AHL sources produce pretty pictures | ||
*We could reuse the basic idea behind their circuit if we need to define a multiple-region response to some stimulus | *We could reuse the basic idea behind their circuit if we need to define a multiple-region response to some stimulus | ||
Revision as of 11:36, 16 June 2006
Pattern formation and quorum sensing
- Basu S, Gerchman Y, Collins CH, Arnold FH, and Weiss R. A synthetic multicellular system for programmed pattern formation. Nature. 2005 Apr 28;434(7037):1130-4. DOI:10.1038/nature03461 |
Basu et al.
- Basu et al created a gene circuit with a non-monotonic response to AHL concentration.
- Main idea: use two concentration boundaries to define three regions with different transcriptional activity
- GFP is repressed at low and high concentrations of AHL, and expressed at medium concentrations
- Basu et al. created three variants with different upper boundaries
- Concentration varies with distance; thus, a mixture of different variants of bacteria produces a bullseye pattern when exposed to a point source of AHL
- Multiple AHL sources produce pretty pictures
- We could reuse the basic idea behind their circuit if we need to define a multiple-region response to some stimulus
