IGEM:Harvard/2006/Brainstorming Papers - Jeffrey: Difference between revisions

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== Pattern formation and quorum sensing ==
== Self organization ==


<biblio>
<biblio>
# pfqs1 pmid=15858574
# so1 pmid=15858574
# pfqs2 pmid=15159530
# so2 pmid=15096621
</biblio>
</biblio>



Revision as of 11:53, 16 June 2006

Self organization

  1. Basu S, Gerchman Y, Collins CH, Arnold FH, and Weiss R. A synthetic multicellular system for programmed pattern formation. Nature. 2005 Apr 28;434(7037):1130-4. DOI:10.1038/nature03461 | PubMed ID:15858574 | HubMed [so1]
  2. Basu S, Mehreja R, Thiberge S, Chen MT, and Weiss R. Spatiotemporal control of gene expression with pulse-generating networks. Proc Natl Acad Sci U S A. 2004 Apr 27;101(17):6355-60. DOI:10.1073/pnas.0307571101 | PubMed ID:15096621 | HubMed [so2]

All Medline abstracts: PubMed | HubMed

A synthetic multicellular system for programmed pattern formation.

  • Basu et al created a gene circuit with a non-monotonic response to AHL concentration.
  • Main idea: use two concentration boundaries to define three regions with different transcriptional activity
    • GFP is repressed at low and high concentrations of AHL, and expressed at medium concentrations
    • Basu et al. created three variants with different upper boundaries
      • Concentration varies with distance; thus, a mixture of different variants of bacteria produces a bullseye pattern when exposed to a point source of AHL
      • Multiple AHL sources produce pretty pictures
  • We could reuse the basic idea behind their circuit if we need to define a multiple-region response to some stimulus