IGEM:Harvard/2006/Brainstorming Papers - Zhipeng: Difference between revisions
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# cyano2 pmid=16102014 | # cyano2 pmid=16102014 | ||
</biblio> | </biblio> | ||
===Kondo et al.=== | |||
*gene cluster KaiABC involved | |||
*’’’no homology to eukarayotic circadian systems’’’ | |||
*Central oscillator controls output patterns; phase of oscillator controlled by input | |||
*Photosynthesis by day, nitrogen fixation by night (2 incompatible in unicellular) | |||
*Used luciferase DNA expressed downstream as a reporter | |||
*Genome 2.7kb in size (e. coli 6.1kb) | |||
*Put luxAB on a psbA plasmid, luxAB luciferase related protein volatile | |||
**See diagram in paper for circuitry | |||
*KaiABC system | |||
**Overexpression of KaiC represses KaiA and KaiB | |||
**Overexpression of KaiA activates KaiBC | |||
**KaiC center of oscillation | |||
*Question TBA: why 24h period? | |||
**Single point mutations in KaiC can change period from 14h to 60h | |||
*Drosophila and Neurospora also have clock models | |||
*Cyanobacteria has no nuclear envelope, can divide 6h/time | |||
*KaiABC analogues only found on archaeobacteria | |||
===Kucho et al.=== | |||
*Purpose of regulation to prepare cell for night in cyanobacteria | |||
*Tested Synechocystis to draw analogies to circadian regulation | |||
*77% in Synechocystis rhymic clones; 100% in cyanobacteria | |||
*In cyanobacteria | |||
**KaiC regulated by negative feedback autoreg. Loop | |||
**KaiA activates kaiBC | |||
*Used DNA microarray to determine mRNA cyclic nature | |||
*cycling genes detected; peak expression from day to night | |||
**Genes mostly: | |||
***unassigned | |||
***carbohydrate metabolism | |||
***energy metabolism purposes | |||
***transcription! | |||
***translation! | |||
*2 Sigma factors have circadian rhythm: sll1689, sll0687 on RNAPol |
Revision as of 05:56, 16 June 2006
Cyanobacteria
- Kondo T and Ishiura M. The circadian clock of cyanobacteria. Bioessays. 2000 Jan;22(1):10-5. DOI:10.1002/(SICI)1521-1878(200001)22:1<10::AID-BIES4>3.0.CO;2-A |
- Takigawa-Imamura H and Mochizuki A. Transcriptional autoregulation by phosphorylated and non-phosphorylated KaiC in cyanobacterial circadian rhythms. J Theor Biol. 2006 Jul 21;241(2):178-92. DOI:10.1016/j.jtbi.2005.11.013 |
- Kutsuna S, Nakahira Y, Katayama M, Ishiura M, and Kondo T. Transcriptional regulation of the circadian clock operon kaiBC by upstream regions in cyanobacteria. Mol Microbiol. 2005 Sep;57(5):1474-84. DOI:10.1111/j.1365-2958.2005.04781.x |
- Kucho K, Okamoto K, Tsuchiya Y, Nomura S, Nango M, Kanehisa M, and Ishiura M. Global analysis of circadian expression in the cyanobacterium Synechocystis sp. strain PCC 6803. J Bacteriol. 2005 Mar;187(6):2190-9. DOI:10.1128/JB.187.6.2190-2199.2005 |
Kondo et al.
- gene cluster KaiABC involved
- ’’’no homology to eukarayotic circadian systems’’’
- Central oscillator controls output patterns; phase of oscillator controlled by input
- Photosynthesis by day, nitrogen fixation by night (2 incompatible in unicellular)
- Used luciferase DNA expressed downstream as a reporter
- Genome 2.7kb in size (e. coli 6.1kb)
- Put luxAB on a psbA plasmid, luxAB luciferase related protein volatile
- See diagram in paper for circuitry
- KaiABC system
- Overexpression of KaiC represses KaiA and KaiB
- Overexpression of KaiA activates KaiBC
- KaiC center of oscillation
- Question TBA: why 24h period?
- Single point mutations in KaiC can change period from 14h to 60h
- Drosophila and Neurospora also have clock models
- Cyanobacteria has no nuclear envelope, can divide 6h/time
- KaiABC analogues only found on archaeobacteria
Kucho et al.
- Purpose of regulation to prepare cell for night in cyanobacteria
- Tested Synechocystis to draw analogies to circadian regulation
- 77% in Synechocystis rhymic clones; 100% in cyanobacteria
- In cyanobacteria
- KaiC regulated by negative feedback autoreg. Loop
- KaiA activates kaiBC
- Used DNA microarray to determine mRNA cyclic nature
- cycling genes detected; peak expression from day to night
- Genes mostly:
- unassigned
- carbohydrate metabolism
- energy metabolism purposes
- transcription!
- translation!
- Genes mostly:
- 2 Sigma factors have circadian rhythm: sll1689, sll0687 on RNAPol