|
|
| Line 3: |
Line 3: |
| **[[/Schematics#2D|2D]] | | **[[/Schematics#2D|2D]] |
| **[[/Schematics#3D|3D]] | | **[[/Schematics#3D|3D]] |
| *[[/Scaffold Sequences]] | | *[[/Scaffold Sequences|Scaffold Sequences]] |
| *[[/Python Code]] | | *[[/Python Code|Python Code]] |
| *[[/Latch Sequences]] | | *[[/Latch Sequences|Latch Sequences]] |
| | | *[[/Oligos|Oligos]] |
| | |
| ===Design details===
| |
| based on ''n'' = 0.324 nm, ''d'' = 3.0 nm
| |
| {| {{table}}
| |
| | align="center" style="background:#f0f0f0;"|'''Shape'''
| |
| | align="center" style="background:#f0f0f0;"|'''Lid ply'''
| |
| | align="center" style="background:#f0f0f0;"|'''Cylinder design'''
| |
| | align="center" style="background:#f0f0f0;"|'''Lid design'''
| |
| | align="center" style="background:#f0f0f0;"|'''Total scaffold length'''
| |
| |-
| |
| | honeycomb hexagon||single||30 tubes (29.0 nm dia) x (84+12)bp long (30.8 nm) = 2880bp||14 tubes (34.2 nm) x (84+12)bp long (30.8 nm) = 1344bp each||2880+(2*1344) + (3*200)bp linkers = 6168 bp
| |
| |}
| |
| | |
| | |
| | |
| ===Sequence Chunks (TChan, 7.5.06)===
| |
| *[[/Oligo_Paths_and_Chunks_TC|Oligo Paths and Chunks (TChan)]] | |
| | |
| ===Sequence Chunks (TChan, 6.27.06)===
| |
| | |
| *[[Media:Chunk1p7308_TC_62706.txt]]
| |
| **Chunk of p7308 sequence for container4's lid1.
| |
| | |
| *[[Media:Chunk2p7308_TC_62706.txt]]
| |
| **Chunk of p8256 sequence for container4's barrel (skips 6bp after lid1's chunk, 6bp right before lid2, the 96bp*13helices that is lid2, 6bp after lid2, and appends the final helix).
| |
| | |
| *[[Media:Chunk3p7308_TC_62706.txt]]
| |
| **Chunk of p7308 sequence for container4's lid2.
| |
| | |
| ===Oligos (TChan, 6.27.06)===
| |
| | |
| *[[Media:lid1_7308_TC_62706.txt]]
| |
| **Oligos for lid 1 (correct wordcount: 30lines, 30words, 1122chars (includes 30 carriage returns) for (96bp-12ssbp)*13helices)
| |
| | |
| *[[Media:barrel_7308_TC_62706.txt]]
| |
| **Oligos for barrel (correct wordcount: 74lines, 74words, 2594chars (includes 74 carriage returns) for (96bp-12ssbp)*30helices)
| |
| | |
| *[[Media:lid2_7308_TC_62706.txt]]
| |
| **Oligos for lid 2 (correct wordcount: 30lines, 30words, 1122chars (includes 30 carriage returns) for (96bp-12ssbp)*13helices)
| |
