IGEM:Harvard/2006/Container Design 4/Python Code: Difference between revisions
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*[[IGEM:Harvard/2006/Container_Design_4/Python_Code/Full_Code_Final|Full Code - Finalized 7/11]]<br> | *[[IGEM:Harvard/2006/Container_Design_4/Python_Code/Full_Code_Final|Full Code - Finalized 7/11]]<br> | ||
*[[IGEM:Harvard/2006/Container_Design_4/Python_Code/Split_Scaffold|Split Scaffold]] | *[[IGEM:Harvard/2006/Container_Design_4/Python_Code/Split_Scaffold|Split Scaffold]] | ||
*[[IGEM:Harvard/2006/Container_Design_4/Python_Code/Split_Oligos|Oligo Splitting - With User Input]]<br> | |||
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Revision as of 07:38, 12 July 2006
Split Oligos From File Input
Script for splitting up oligos automatically (FILE INPUT). (make sure to import pickle at the top). This part goes in main. Further down is the file to read from essentially (uses pickle)
##### # Oligo splitting - this time reading from a file and not asking for user # input ##### fin_barrel = None fin_lid = None try: fin_barrel = open("barrel_oligos_to_split.txt", "r") fin_lid = open("lid_oligos_to_split.txt", "r") except IOError, e: print "Error in file IO: ", e # Ask the user if they are running a lid or a barrel shape = int(raw_input("Enter 1 if you are running a barrel, 2 if lid: ")) if (shape == 1): oligos_to_split = pickle.load(fin_barrel) elif (shape == 2): oligos_to_split = pickle.load(fin_lid) else: print 'Please modify code or run with lid or 30hb barrel' new_OTP_ra = OTP_ra[:] for pair in oligos_to_split: oligo_num = pair[0] print oligo_num num_toks = pair[1] print num_toks new_OTP_ra = split_oligo(new_OTP_ra, oligo_num, num_toks) print new_OTP_ra print len(OTP_ra) print len(new_OTP_ra) # if it's the barrel design and so that all the numbers aren't messed # up, the 7bp token on the start of strand 10 needs to be removed # because it's going to be left unpaired if (shape == 1): new_OTP_ra = new_OTP_ra[:61] + new_OTP_ra[62:] if fin_barrel: fin_barrel.close() if fin_lid: fin_lid.close()
Pickle Split Parameters
### split = [[oligo_num, num_tokens for oligo_1 - from 5prime], ... ] import pickle fout_barrel = None fout_lid = None try: fout_barrel = open("barrel_oligos_to_split.txt", "w") fout_lid = open("lid_oligos_to_split.txt", "w") except IOError, e: print "Error in file IO: ", e barrel_split = [[56, 2], [57, 3], [41, 3], [26, 3], [21, 4]] lid_split = [[27, 4], [2, 4]] pickle.dump(barrel_split, fout_barrel) pickle.dump(lid_split, fout_lid) # clean up if they're open if fout_barrel: fout_barrel.close() if fout_lid: fout_lid.close()
Add aptamers
####### # Add aptamers to the ends of the appropriate oligos. ####### # Constants apt_seq = 'GGTTGGTGTGGTTGG' T_linker = 'TTT' print oligo_ra num_aptamers = int(raw_input('How many aptamers do you want to add? ')) i = 0 while i < num_aptamers: oligo_num = int(raw_input('Which oligo needs an aptamer? ')) if oligo_num < len(oligo_ra): # Add the aptamer to that oligo oligo_ra[oligo_num] = oligo_ra[oligo_num] + T_linker + apt_seq i = i + 1 else: print 'oligo ' + str(oligo_num) + ' out of range.' print oligo_ra
Add aptamers using File Input rather than User Input
##### # Add apts this time using file input instead of user input ##### # Constants apt_seq = 'GGTTGGTGTGGTTGG' T_linker = 'TTT' fin_barrel = None try: fin_barrel = open("barrel_apts_to_add.txt", "r") except IOError, e: print "Error in file IO: ", e # Ask the user if they are running a lid or a barrel shape = int(raw_input("Enter 1 if you are running a barrel, 2 if lid: ")) if (shape == 1): apts_to_add = pickle.load(fin_barrel) for apt_specs in apts_to_add: oligo_num = apt_specs[0] type = apt_specs[1] if (type == 1): # apt is pointing in so add 'I' as a flag at the end oligo_ra[oligo_num] = oligo_ra[oligo_num] + T_linker + apt_seq + 'I' elif(type == 2): # apt is pointing out so add 'O' as a flag oligo_ra[oligo_num] = oligo_ra[oligo_num] + T_linker + apt_seq + 'O' else: # incorrect type print 'Bad input - aptamer needs to be pointing in or out'
Print Aptamer Oligos
Oligo sorting - find an print out those with aptamers
##### # oligo sorting ##### # sort based on whether or not there's an aptamer attached to the end of # an oligo apt = re.compile('TTTGGTTGGTGTGGTTGG') oligo_num = 0 for oligo in oligo_ra: m = apt.search(oligo) if m: print 'Match found: ', oligo + ' : ' + str(oligo_num) else: print 'No match' + str(oligo_num) oligo_num = oligo_num + 1
Modifications to honeycomb_v1 scripts
- Modifications to William's program to print each oligo number next to what tokens it represents
oligo_num = 0 for oligo in OTP_ra: for token in oligo: print str(oligo_num) + ": ", token oligo_num = oligo_num + 1
- Modifications to William's program to print a grid of oligo numbers completely filled in
- add this part to main (AAA or BBB)
#### # generate and print the oligo grid #### # Initialize the grid with all periods num_strands = len(TPP_ra) num_subzones = len(TPP_ra[0]) sub_token_visit_ra = ['.' for subzone_num in range(num_subzones)] grid_ra = [sub_token_visit_ra[:] for strand_num in range(num_strands)] oligo_num = 0 for oligo in OTP_ra: grid_ra = generate_oligo_path(oligo, oligo_num, grid_ra) oligo_num = oligo_num + 1 print grid_ra print_all_oligos(grid_ra, num_strands, num_subzones)
- add this part to honeycomb_pointers_v1.py
# The idea here is to have a function that adds the numbers of one oligo path # to the appropriate places in the big grid array. Eventually this will be printed # in main. Also it needs to be initialized in main. Oligo_path is the path of # one oligo, while grid_ra is the grid that is constantly being updated until # it is printed in main. oligo_num is number that will be inputed to the grid_ra. def generate_oligo_path(oligo_path, oligo_num, grid_ra): num_path_tokens = len(oligo_path) # Assign visits for path_token_num in range(num_path_tokens): token = oligo_path[path_token_num] strand = token[0] subzone = token[1] grid_ra[strand][subzone] = oligo_num return grid_ra def print_all_oligos(grid_ra, num_strands, num_subzones): spacer = ' ' for strand_num in range(num_strands): for subzone_num in range(num_subzones): visitor_string = str(grid_ra[strand_num][subzone_num]) sys.stdout.write(visitor_string) sys.stdout.write(spacer[:4 - len(visitor_string)]) sys.stdout.write('\n')