IGEM:Harvard/2006/Cyanobacteria: Difference between revisions

Revision as of 16:34, 17 June 2006

Think about these questions when preparing your project proposals for the group meeting.

For each project idea:

What is the specific goal of the project?
- Biobrick the oscillator
  - Biobrick kaiA, kaiBC.
  - need to find out how kaiA/BC are regulated to do so
- Test the oscillator in E. coli
- Alternate phrasing, courtesy of Kit Parker - what is the "deliverable?" The thing you will point to and say "this is our project?"
  - Our deliverable is a (multiple?) BioBrick part(s)
What are two or three possible means of implementing the idea?
Risk
- How many untested things have to work for the project to succeed?
- How will you test whether those things work or not?
- How will you adjust your plan when one of these things fails to work?
- How will you minimize the time/effort/resources lost to a failed design?
  - Can your time/effort/resources apply to more than one design simultaneously?
Reward
- How cool, fun, exciting is the project for you?
- What if any is the usefulness or societal benefit of the project?
- What is going to impress the judges in November?
Timeline
- What are the project milestones? (design, construction, testing)
- What is the estimated time required for each? (always overestimate)
- If you can't reach your ultimate goal by August, is there a satisfying intermediate goal?
- What is the immediate next step in pursuing the project?
  - If DNA synthesis will be required, how soon will you have the sequence designed?

Nakajima et. al: in vitro, the proteins oscillate abeit not with as large of an amplitude

@@ Line 11: / Line 11: @@
 **'''Test the oscillator in E. coli'''
 ** Alternate phrasing, courtesy of Kit Parker - what is the "deliverable?" The thing you will point to and say "this is our project?"
-***'''Our deliverable is a (multiple?) BioBrick parts
+***'''Our deliverable is a (multiple?) BioBrick part(s)
 * What are two or three possible means of implementing the idea?
 * Risk