IGEM:Harvard/2006/Cyanobacteria: Difference between revisions

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<ul>
<li id="current">[[IGEM:Harvard/2006/Cyanobacteria|1. Main Page]]</li>
<li id="current">[[IGEM:Harvard/2006/Cyanobacteria|1. Main Page]]</li>
<li>[[IGEM:Harvard/2006/Cyanobacteria_Lab_Notebook|2. Lab Notebook]]</li>
<li>[[IGEM:Harvard/2006/Cyanobacteria/Notebook|2. Lab Notebook]]</li>
<li>[[IGEM:Harvard/2006/Cyanobacteria_Literature|3. Literature]]</li>
<li>[[IGEM:Harvard/2006/Cyanobacteria/Literature|3. Literature]]</li>
<li>[[IGEM:Harvard/2006/Cyanobacteria_Incubator|4. Incubator]]</li>
<li>[[IGEM:Harvard/2006/Cyanobacteria/Incubator|4. Incubator]]</li>
<li>[[IGEM:Harvard/2006/Cyanobacteria_Care|5. Care]]</li>
<li>[[IGEM:Harvard/2006/Cyanobacteria/Care|5. Care]]</li>
<li>[[IGEM:Harvard/2006/Cyanobacteria_Contacts|6. Contacts]]</li>
<li>[[IGEM:Harvard/2006/Cyanobacteria/Contacts|6. Contacts]]</li>
</ul>
</ul>
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Revision as of 10:32, 7 July 2006


Agenda

See image at right for our long-term project outline.

Long-term project outline

For Monday 6/26

  • Figure out optimal growing conditions (complete)
  • Regrow cyanobacteria colonies (complete)
  • Draft email questions for Professor Golden (complete)

For Tuesday 6/27

  • PCR crash course (complete)
  • Picnic! (complete)
  • Check first batch of colonies again (complete)
  • Complete more research in preparation for Professor Golden (complete)
  • Order primers (complete)

For Wednesday 6/28

  • Research transformation protocols (mostly complete)
  • Start creating presentation for Friday
  • Talk to Professor Golden (complete)
  • Grow additional liquid cyanobacteria cultures (new order of PCC 7942 is not here yet)
  • Finalize goals (work in cyanobacteria or in e. coli)/ plan out the rest of summer (in progress)

For Thursday 6/29

  • Research Sigma factors (on hold)
  • Design and order our first set of primers (for entire KaiABC sequence) (complete)
  • Make presentation for Friday (complete)

For Friday 6/30

  • Present before Fourth of July weekend (cancelled)

For Wednesday 7/5

  • Research plasmids to ask Prof. Golden (moved from Thursday)
    • Also, don't forget to ask her about CO2 and aerating our cultures
  • Find out what equipment we would need for measuring bioluminescence

For Thursday 7/6

  • Make a model of the cyanobacteria
    • We missed ice cream at Maxwell-Dworkin

For Friday 7/7

  • Make a model of the cyanobacteria
  • We should be receiving the shipment of PCC 7942, otherwise we will call ATCC for the tracking information
    • PCR PCC7942
    • Plate PCC7942
    • Grow PCC7942 in liquid culture, both with thiosulfate and without
  • TGIF in Bauer Center

Protocols

Main article: IGEM:Harvard/2006/Protocols/Cyanobacteria

Presentations

Working Team Members


Notebook

<calendar> name=/Notebook date=2006/07/01 view=threemonths format=%name/%year-%month-%day weekstart=14 </calendar> For each page, newer entries are at the top.