IGEM:Harvard/2006/Cyanobacteria

• 1. Main Page
• 2. Lab Notebook
• 3. Literature
• 4. Incubator
• 5. Protocols
• 6. Care
• 7. Contacts

Today's date: Tuesday, April 16, 2024

Agenda

See image at right for our long-term project outline.

Long-term project outline

For Monday 6/26

• Figure out optimal growing conditions (complete)
• Regrow cyanobacteria colonies (complete)
• Draft email questions for Professor Golden (complete)

For Tuesday 6/27

• PCR crash course (complete)
• Picnic! (complete)
• Check first batch of colonies again (complete)
• Complete more research in preparation for Professor Golden (complete)
• Order primers (complete)

For Wednesday 6/28

• Research transformation protocols (mostly complete)
• Start creating presentation for Friday
• Talk to Professor Golden (complete)
• Grow additional liquid cyanobacteria cultures (new order of PCC 7942 is not here yet)
• Finalize goals (work in cyanobacteria or in e. coli)/ plan out the rest of summer (in progress)

For Thursday 6/29

• Research Sigma factors (on hold)
• Design and order our first set of primers (for entire KaiABC sequence) (complete)
• Make presentation for Friday (complete)

For Friday 6/30

• Present before Fourth of July weekend (cancelled)

For Wednesday 7/5

• Research plasmids to ask Prof. Golden (moved from Thursday)
  • Also, don't forget to ask her about CO2 and aerating our cultures
• Find out what equipment we would need for measuring bioluminescence

For Thursday 7/6

• Make a model of the cyanobacteria
  • We missed ice cream at Maxwell-Dworkin

For Friday 7/7

• Make a model of the cyanobacteria
• We should be receiving the shipment of PCC 7942, otherwise we will call ATCC for the tracking information
  • PCR PCC7942
  • Plate PCC7942
  • Grow PCC7942 in liquid culture, both with thiosulfate and without
• TGIF in Bauer Center

Presentations

• Project proposal (week 2)
• File:Cyan week3.ppt
  • Built incubator and obtained WH8102, PCC7942, and PCC6803 strains

Working Team Members

• Hetmann Hsieh (talk)
• Jeffrey Lau (talk)
• Zhipeng Sun (talk)
• David Ramos (talk)