IGEM:Harvard/2006/Cyanobacteria: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
JeffreyLau (talk | contribs) |
||
| Line 7: | Line 7: | ||
<br style="clear:both"> | <br style="clear:both"> | ||
=== Lengths === | |||
From VF2 to VR (BioBrick primers): | |||
* KaiA + J04500: 1406 bp | |||
* KaiB + J04500: 859 bp | |||
* KaiC + J04500: 2110 bp | |||
==Synthesis== | ==Synthesis== | ||
Revision as of 02:06, 30 August 2006
<html><style type='text/css'> .tabs {
font-size:80%;
font-weight:none;
width: 100%;
color: #FFFFFF;
background:#FFFFFF url("/images/5/54/DarkgreenTab-bg.gif") repeat-x bottom;
}
.tabs li {
background:url("/images/3/36/DarkgeenTab-left.gif") no-repeat left top;
}
.tabs a,.tabs strong {
background:url("/images/d/d3/DarkgreenTab-right.gif") no-repeat right top;
color:#FFFFFF;
padding: 3px 10px 3px 4px;
}
.tabs strong{
color:#CCFF00;
background-image:url("/images/b/b1/DarkgreenTab-right_on.gif");
}
.tabs a:hover{
color:#66FF00;
}
</style></html>
Construct Planning
Lengths
From VF2 to VR (BioBrick primers):
- KaiA + J04500: 1406 bp
- KaiB + J04500: 859 bp
- KaiC + J04500: 2110 bp
Synthesis
Agenda
See image at right for our long-term project outline.
Click here for our current agenda
Kai Gene sizes
- KaiA size: 855bp
- 903bp with BioBrick ends
- KaiB: 309bp
- 357bp with BioBrick ends
- KaiC: 1560bp
- KaiA size: 855bp
BioBricks Used
- <bbpart>BBa_J04450</bbpart>
- RFP device
- Insert size: 1069bp
- [pSB1A2]
- High-copy, AmpR
- Size: 2079bp
- <bbpart>BBa_J04500</bbpart>
- Lac promoter + RBS
- Insert size: 220bp
- [pSB1AK3]
- High-copy, AmpR, KanR
- Insert size: 3189bp
- [pSB4A3]
- Low-copy, AmpR
- Insert size: 3339 bp
- <bbpart>BBa_R0010</bbpart> + <bbpart>BBa_E0241</bbpart>
- GFP device
- Insert size: 995 bp
- <bbpart>BBa_J04450</bbpart>
Presentations
- Project proposal (week 2)
- Week 3 progress update
- Built incubator and obtained WH8102, PCC7942, and PCC6803 strains
- Week 4 progress update
- Week 5 progress update, upd. 10:10 7/17
- Week 6 progress update, upd. 10:02 7/24 HH
- Week 7 progress update
- Week 8 progress update
- Week 9 progress update
- Week 10 progress update, 50% complete
- Final Presentation (incomplete)
- Final Presentation (complete)
Team Members
- Hetmann Hsieh (talk, edits)
- Jeffrey Lau (talk, edits)
- Zhipeng Sun (talk, edits)
- David Ramos (talk, edits)
Recent Changes
List of abbreviations:
- N
- This edit created a new page (also see list of new pages)
- m
- This is a minor edit
- b
- This edit was performed by a bot
- (±123)
- The page size changed by this number of bytes
25 April 2024
|
|
00:22 | The paper that launched microfluidics - Xi Ning 7 changes history +4,723 [Xning098 (7×)] | |||
|
|
00:22 (cur | prev) −97 Xning098 talk contribs | ||||
|
|
00:18 (cur | prev) +14 Xning098 talk contribs (→Summary) | ||||
|
|
00:11 (cur | prev) +1 Xning098 talk contribs (→Introduction) | ||||
|
|
00:11 (cur | prev) +1 Xning098 talk contribs (→Introduction) | ||||
|
|
00:11 (cur | prev) 0 Xning098 talk contribs (→References) | ||||
|
|
00:08 (cur | prev) +4 Xning098 talk contribs (→Significance) | ||||
|
|
00:07 (cur | prev) +4,800 Xning098 talk contribs | ||||
24 April 2024
|
|
18:35 | User:Yanbin Huang 2 changes history +25 [Yanbin Huang (2×)] | |||
|
|
18:35 (cur | prev) +13 Yanbin Huang talk contribs (→Peer-Reviewed Publications) | ||||
|
|
18:34 (cur | prev) +12 Yanbin Huang talk contribs (→Peer-Reviewed Publications) | ||||
|
|
17:49 | Hu 2 changes history +28 [Hugangqing (2×)] | |||
|
|
17:49 (cur | prev) +18 Hugangqing talk contribs | ||||
|
|
17:48 (cur | prev) +10 Hugangqing talk contribs | ||||
|
|
08:14 | "Pick and Place" Assembly of Parts Using PDMS - Amy Lim, Rylie Costello 6 changes history +394 [Rcostello (6×)] | |||
|
|
08:14 (cur | prev) +1 Rcostello talk contribs (→"Pick and Place" Methodology) | ||||
|
|
08:13 (cur | prev) −14 Rcostello talk contribs (→"Pick and Place" Methodology) | ||||
|
|
08:12 (cur | prev) −1 Rcostello talk contribs (→"Pick and Place" Methodology) | ||||
|
|
08:12 (cur | prev) −1 Rcostello talk contribs (→"Pick and Place" Methodology) | ||||
|
|
08:12 (cur | prev) +110 Rcostello talk contribs (→References) | ||||
|
|
08:11 (cur | prev) +299 Rcostello talk contribs (→"Pick and Place" Methodology) | ||||
| 08:02 | Upload log Rcostello talk contribs uploaded File:Pick and Place.mp4 | ||||
23 April 2024
|
|
15:33 | "Pick and Place" Assembly of Parts Using PDMS - Amy Lim, Rylie Costello 6 changes history +837 [Rcostello (6×)] | |||
|
|
15:33 (cur | prev) +1 Rcostello talk contribs (→"Pick and Place" for Microfluidics) | ||||
|
|
15:33 (cur | prev) +203 Rcostello talk contribs (→References) | ||||
|
|
15:31 (cur | prev) −2 Rcostello talk contribs (→"Pick and Place" for Microfluidics) | ||||
|
|
15:29 (cur | prev) −474 Rcostello talk contribs (→References) | ||||
|
|
15:29 (cur | prev) +845 Rcostello talk contribs (→MEMS Devices) | ||||
|
|
15:14 (cur | prev) +264 Rcostello talk contribs (→"Pick and Place" for Microfluidics) | ||||
|
|
11:58 | BioMicroCenter:People 2 changes history +30 [Lttran (2×)] | |||
|
|
11:58 (cur | prev) −4 Lttran talk contribs (→BioMicro Center Staff) | ||||
|
|
11:49 (cur | prev) +34 Lttran talk contribs (→BioMicro Center Staff) | ||||
| 11:46 | Upload log Lttran talk contribs uploaded File:SKR BMC.jpg | ||||
22 April 2024
|
|
19:28 | "Pick and Place" Assembly of Parts Using PDMS - Amy Lim, Rylie Costello 2 changes history −2 [Rcostello (2×)] | |||
|
|
19:28 (cur | prev) −2 Rcostello talk contribs (→Nanowires) | ||||
|
|
19:26 (cur | prev) 0 Rcostello talk contribs (→Biology-Inspired Solution) | ||||
