IGEM:Harvard/2006/Cyanobacteria

• 1. Main Page
• 2. Lab Notebook
• 3. Literature
• 4. Incubator
• 5. Protocols
• 6. Care
• 7. Contacts
• 8. Primers

Today's date: Friday, April 26, 2024

Agenda

See image at right for our long-term project outline.

Long-term project outline

Monday 6/26

• Figure out optimal growing conditions (complete)
• Regrow cyanobacteria colonies (complete)
• Draft email questions for Professor Golden (complete)

Tuesday 6/27

• PCR crash course (complete)
• Picnic! (complete)
• Check first batch of colonies again (complete)
• Complete more research in preparation for Professor Golden (complete)
• Order primers (complete)

Wednesday 6/28

• Research transformation protocols (mostly complete)
• Start creating presentation for Friday
• Talk to Professor Golden (complete)
• Grow additional liquid cyanobacteria cultures (new order of PCC 7942 is not here yet)
• Finalize goals (work in cyanobacteria or in e. coli)/ plan out the rest of summer (in progress)

Thursday 6/29

• Research Sigma factors (on hold)
• Design and order our first set of primers (for entire KaiABC sequence) (complete)
• Make presentation for Friday (complete)

Friday 6/30

• Present before Fourth of July weekend (cancelled)

Wednesday 7/5

• Research plasmids to ask Prof. Golden (moved from Thursday)
  • Also, don't forget to ask her about CO2 and aerating our cultures
• Find out what equipment we would need for measuring bioluminescence

Thursday 7/6

• Make a model of the cyanobacteria
• We missed ice cream at Maxwell-Dworkin

Friday 7/7

• Make a model of the cyanobacteria (complete)
• We should be receiving the shipment of PCC 7942, otherwise we will call ATCC for the tracking information
  • PCR PCC7942 (complete-- turned out negative)
  • Plate PCC7942 (complete)
  • Grow PCC7942 in liquid culture, both with thiosulfate and without (complete)
• TGIF in Bauer Center (complete)

Monday 7/10

• Make frozen stocks of our new grown PCC 7942 (complete)
• Research PCR protocols for cyanobacteria (maybe?) (complete)
• PCR (complete)
• Research DNA synthesis companies (complete)
• Design sequence to be synthesized (incomplete)
• Research homologous recombination design (incomplete)

Presentations

• Project proposal (week 2)
• Week 3 progress update
  • Built incubator and obtained WH8102, PCC7942, and PCC6803 strains
• Week 4 progress update

Team Members

• Hetmann Hsieh (talk)
• Jeffrey Lau (talk)
• Zhipeng Sun (talk)
• David Ramos (talk)