IGEM:Harvard/2006/Cyanobacteria

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Construct Planning

Constructs we plan to create.

Synthesis

Synthesis

Agenda

See image at right for our long-term project outline.

Long-term project outline

Click here for our current agenda

Kai Gene sizes

• KaiA size: 855bp
  • 903bp with BioBrick ends
• KaiB: 309bp
  • 357bp with BioBrick ends
• KaiC: 1560bp

BioBricks Used

• <bbpart>BBa_J04450</bbpart>
  • RFP device
  • Insert size: 1069bp
  • [pSB1A2]
    • High-copy, Amp^R
    • Size: 2079bp
• <bbpart>BBa_J04500</bbpart>
  • Lac promoter + RBS
  • Insert size: 220bp
  • [pSB1AK3]
    • High-copy, Amp^R, Kan^R
    • Insert size: 3189bp
• [pSB4A3]
  • Low-copy, Amp^R
  • Insert size: 3339 bp
• <bbpart>BBa_R0010</bbpart> + <bbpart>BBa_E0241</bbpart>
  • GFP device
  • Insert size: 995 bp

Presentations

• Project proposal (week 2)
• Week 3 progress update
  • Built incubator and obtained WH8102, PCC7942, and PCC6803 strains
• Week 4 progress update
• Week 5 progress update, upd. 10:10 7/17
• Week 6 progress update, upd. 10:02 7/24 HH
• Week 7 progress update
• Week 8 progress update
• Week 9 progress update

Team Members

• Hetmann Hsieh (talk, edits)
• Jeffrey Lau (talk, edits)
• Zhipeng Sun (talk, edits)
• David Ramos (talk, edits)

Recent Changes