IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-10-2
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Re-Digestions eg. peng is dumb
Previously, the B0034 should have been digested with \SP, and KaiC with \XP. Not sure what I was thinking there...
- B0034 \SP + J36000 KaiC \XP
- B0034 \SP + J36001 KaiB \XP
(Note: Just KaiA, B, or C on a backbone we can submit the geneart plasmids; as far as i can tell they do not have other E/X/S/P sites)
It looks like we have a signifigant amount of J36000 KaiC \XP.'
Then:
- B0034 \SP: 8 lanes
- J36001 \XP: 8 lanes
//Why 6? Fits perfectly into a 20 well gel!
Protocols:
B0034 \SP: 6 samples
- 7.6 uL DNA (46.36)
- 1 µL 10 x BSA (6.1)
- 1 µL 10x buffer #2 (6.1)
- 0.2 µL Spe1 (1.22)
- 0.2 µL Pst1 (1.22)
J36001 \XP: 6 samples
- 7.6 uL DNA (46.36)
- 1 µL 10 x BSA (6.1)
- 1 µL 10x buffer #3 (6.1)
- 0.2 µL Xba1 (1.22)
- 0.2 µL Pst1 (1.22)
The usual, @ 16h @45C.