IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-10-23

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Revision as of 14:05, 23 October 2006 by Zsun (talk | contribs) (→‎post-stain)
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Redo of yesterday's procedure

CIP of digested 011/sp

1h@37C

gel of post-CIP'd

1h15@130V. 1.2%

  • 1: 1kb+
  • 2: undigested 11
  • 3: empty
  • 4: 11/sp marker
  • 5: 11/sp (not shown)
  • 6: 11/sp (not shown)
  • 7: 11/sp (not shown)
  • 8: 11/sp (not shown)
  • 9: 11/sp marker
  • 10: 1kb+

post-stain

Very carcinogenic :D 40uL EtBr, 20min

see above for legend
verification correct band cutout


gel purification and pcr purification

2000mg gel; purified into 6 tubes. sent all 6 tubes into 1 pcr purification. eluted in 30 eb.

sequencing

Sent 4 orders: 11 Vf2&vr and 22 vf2&vr, rush order.

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