IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-10-23
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Redo of yesterday's procedure
CIP of digested 011/sp
1h@37C
gel of post-CIP'd
1h15@130V. 1.2%
- 1: 1kb+
- 2: undigested 11
- 3: empty
- 4: 11/sp marker
- 5: 11/sp (not shown)
- 6: 11/sp (not shown)
- 7: 11/sp (not shown)
- 8: 11/sp (not shown)
- 9: 11/sp marker
- 10: 1kb+
post-stain
Very carcinogenic :D 40uL EtBr, 20min
gel purification and pcr purification
2000mg gel; purified into 6 tubes. sent all 6 tubes into 1 pcr purification. eluted in 30 eb.
sequencing
Sent 4 orders: 11 Vf2&vr and 22 vf2&vr, rush order.