IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-10-24: Difference between revisions
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==Results of transformation== | |||
Plate with many cells on it; picked 8 to PCR (see 2 days ago for PCR protocol, except extension time decreased to 3 min). Expected value is ~2650bp. | |||
Also restreaked; marked in chinese numerals :D in the fridge. | |||
===SUCCESS!=== | ===SUCCESS!=== | ||
| Line 4: | Line 9: | ||
*Chose colony 6 (liu). | *Chose colony 6 (liu). | ||
==Review== | |||
We have (based on old numbering system): | |||
*J36000: KaiC on geneart plasmid | |||
**Sequenced | |||
*J36001: KaiB on geneart plasmid | |||
**Sequenced | |||
*J36002: KaiA on geneart plasmid | |||
**Sequenced | |||
*J36010: Lac + RBS + KaiC (eg. J4500/sp + J36000/xp) | |||
**Sequenced | |||
**Frozen stock called "M2-7" | |||
**Kan / Amp | |||
*J36011: Lac + RBS + KaiB (eg. J4500/sp + J36001 /xp) | |||
**Sequencing submitted, pending | |||
**Frozen stock called "colony E" | |||
**Kan / Amp | |||
*J36012: Lac + RBS + KaiA (eg. J4500/sp + J36001 /xp) | |||
**Sequenced | |||
**Frozen stock called "A11" (I think, though I need to double check) | |||
**Kan / Amp | |||
*J36021: J36011/sp + J36010 /xp | |||
**Sequencing not yet submitted | |||
**Frozen stock not yet made | |||
**Picked colony liu (6). | |||
**Kan / Amp | |||
*J36022: J36012/sp + J36010 /xp | |||
**Sequencing submitted, pending | |||
**Frozen stock called "colony 1" | |||
**Kan / Amp | |||
*J04430: GFP device behind Lac | |||
**Nick made, demonstrated works | |||
**ONLY Amp | |||
Revision as of 16:39, 24 October 2006
Results of transformation
Plate with many cells on it; picked 8 to PCR (see 2 days ago for PCR protocol, except extension time decreased to 3 min). Expected value is ~2650bp.
Also restreaked; marked in chinese numerals :D in the fridge.
SUCCESS!
- Chose colony 6 (liu).
Review
We have (based on old numbering system):
- J36000: KaiC on geneart plasmid
- Sequenced
- J36001: KaiB on geneart plasmid
- Sequenced
- J36002: KaiA on geneart plasmid
- Sequenced
- J36010: Lac + RBS + KaiC (eg. J4500/sp + J36000/xp)
- Sequenced
- Frozen stock called "M2-7"
- Kan / Amp
- J36011: Lac + RBS + KaiB (eg. J4500/sp + J36001 /xp)
- Sequencing submitted, pending
- Frozen stock called "colony E"
- Kan / Amp
- J36012: Lac + RBS + KaiA (eg. J4500/sp + J36001 /xp)
- Sequenced
- Frozen stock called "A11" (I think, though I need to double check)
- Kan / Amp
- J36021: J36011/sp + J36010 /xp
- Sequencing not yet submitted
- Frozen stock not yet made
- Picked colony liu (6).
- Kan / Amp
- J36022: J36012/sp + J36010 /xp
- Sequencing submitted, pending
- Frozen stock called "colony 1"
- Kan / Amp
- J04430: GFP device behind Lac
- Nick made, demonstrated works
- ONLY Amp
