IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-10-27: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
JeffreyLau (talk | contribs) (→Washes) |
JeffreyLau (talk | contribs) No edit summary |
||
| Line 1: | Line 1: | ||
== Secondary antibody incubation == | == Secondary antibody incubation == | ||
Incubated for 45 minutes, then | Incubated for 45 minutes, then transferred the liquid to tube labeled "IRDye 700" for reuse. | ||
== Final wash schedule == | == Final wash schedule == | ||
Revision as of 07:58, 27 October 2006
Secondary antibody incubation
Incubated for 45 minutes, then transferred the liquid to tube labeled "IRDye 700" for reuse.
Final wash schedule
- 2 10m washes of PBST
- 2 10m washes of PBS
- Rinse with distilled water when finished
- Wrap in aluminum foil, store in 4C refrigerator until imaging
