IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-10-27: Difference between revisions
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Latest revision as of 10:45, 27 October 2006
Secondary antibody incubation
Incubated for 45 minutes, then transferred the liquid to tube labeled "IRDye 700" for reuse.
Final wash schedule
- 2 10m washes of PBST
- First wash started: 1050
- Second wash started: 1216
- 2 10m washes of PBS
- First wash started: 1230
- Second wash started: 1243 --note: we washed for only 5m due to time constraints
- Rinse with distilled water when finished
- Wrap in aluminum foil, store in 4C refrigerator until imaging
Anybody want some data?
