(Difference between revisions)
Revision as of 10:44, 27 October 2006
Secondary antibody incubation
Incubated for 45 minutes, then pipetted back into tube labeled "IRDye 700" for reuse.
- 2 10m washes of PBST
- 2 10m washes of PBS
- Rinse with distilled water when finished
- Wrap in aluminum foil