IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-10-27

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(Washes)
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Incubated for 45 minutes, then pipetted back into tube labeled "IRDye 700" for reuse.
Incubated for 45 minutes, then pipetted back into tube labeled "IRDye 700" for reuse.
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== Washes ==
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== Final wash schedule ==
* 2 10m washes of PBST
* 2 10m washes of PBST
* 2 10m washes of PBS
* 2 10m washes of PBS
* Rinse with distilled water when finished
* Rinse with distilled water when finished
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* Wrap in aluminum foil
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* Wrap in aluminum foil, store in 4C refrigerator until imaging

Revision as of 09:55, 27 October 2006

Secondary antibody incubation

Incubated for 45 minutes, then pipetted back into tube labeled "IRDye 700" for reuse.

Final wash schedule

  • 2 10m washes of PBST
  • 2 10m washes of PBS
  • Rinse with distilled water when finished
  • Wrap in aluminum foil, store in 4C refrigerator until imaging
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