IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-10-27

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(Final wash schedule)
(Final wash schedule)
Line 4: Line 4:
== Final wash schedule ==
== Final wash schedule ==
* 2 10m washes of PBST
* 2 10m washes of PBST
-
**''First wash started: 1050''
+
** ''First wash started: 1050''
-
**''Second wash started: 1216''
+
** ''Second wash started: 1216''
* 2 10m washes of PBS
* 2 10m washes of PBS
 +
** ''First wash started: 1230''
* Rinse with distilled water when finished
* Rinse with distilled water when finished
* Wrap in aluminum foil, store in 4C refrigerator until imaging
* Wrap in aluminum foil, store in 4C refrigerator until imaging

Revision as of 12:31, 27 October 2006

Secondary antibody incubation

Incubated for 45 minutes, then transferred the liquid to tube labeled "IRDye 700" for reuse.

Final wash schedule

  • 2 10m washes of PBST
    • First wash started: 1050
    • Second wash started: 1216
  • 2 10m washes of PBS
    • First wash started: 1230
  • Rinse with distilled water when finished
  • Wrap in aluminum foil, store in 4C refrigerator until imaging
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