IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-10-28
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| + | == Western blot images == | ||
Western blot images without the annoying rectangle: | Western blot images without the annoying rectangle: | ||
| - | [[Image:cyano_WB_CN_3.jpg|thumb]] | + | [[Image:cyano_WB_CN_3.jpg|thumb|left]] |
| + | [[Image:cyano_WB_CN_3.tif|thumb|left]] | ||
| + | <br style="clear:both";/> | ||
| - | + | == Miniprep of J36335 (KaiA + KaiC), J36832 (KaiB) == | |
| + | We performed 8 minipreps each of J36335 and J36832, eluted in 30 µL EB. | ||
| + | |||
| + | == Digest of J36335\S-P (KaiA + KaiC), J36832\X-P (KaiB) == | ||
| + | We did 8 digests each of J36335\S-P and J36832\X-P. | ||
| + | |||
| + | === Reaction === | ||
| + | J36335\S-P | ||
| + | * 7.6 µL DNA | ||
| + | * 1 µL 10x BSA | ||
| + | * 1 µL NEBuffer 2 | ||
| + | * 0.2 µL SpeI | ||
| + | * 0.2 µL PstI | ||
| + | |||
| + | J36832\X-P | ||
| + | * 7.6 µL DNA | ||
| + | * 1 µL 10x BSA | ||
| + | * 1 µL NEBuffer 3 | ||
| + | * 0.2 µL XbaI | ||
| + | * 0.2 µL PstI | ||
| + | |||
| + | === Master mixes === | ||
| + | J36335\S-P | ||
| + | * 76 µL DNA | ||
| + | * 10 µL 10x BSA | ||
| + | * 10 µL NEBuffer 2 | ||
| + | * 2 µL SpeI | ||
| + | * 2 µL PstI | ||
| + | Aliquot 10µL into each tube. | ||
| + | |||
| + | J36832\X-P | ||
| + | * 76 µL DNA | ||
| + | * 10 µL 10x BSA | ||
| + | * 10 µL NEBuffer 3 | ||
| + | * 2 µL XbaI | ||
| + | * 2 µL PstI | ||
| + | Aliquot 10µL into each tube. | ||
Revision as of 12:30, 28 October 2006
Contents |
Western blot images
Western blot images without the annoying rectangle:
Miniprep of J36335 (KaiA + KaiC), J36832 (KaiB)
We performed 8 minipreps each of J36335 and J36832, eluted in 30 µL EB.
Digest of J36335\S-P (KaiA + KaiC), J36832\X-P (KaiB)
We did 8 digests each of J36335\S-P and J36832\X-P.
Reaction
J36335\S-P
- 7.6 µL DNA
- 1 µL 10x BSA
- 1 µL NEBuffer 2
- 0.2 µL SpeI
- 0.2 µL PstI
J36832\X-P
- 7.6 µL DNA
- 1 µL 10x BSA
- 1 µL NEBuffer 3
- 0.2 µL XbaI
- 0.2 µL PstI
Master mixes
J36335\S-P
- 76 µL DNA
- 10 µL 10x BSA
- 10 µL NEBuffer 2
- 2 µL SpeI
- 2 µL PstI
Aliquot 10µL into each tube.
J36832\X-P
- 76 µL DNA
- 10 µL 10x BSA
- 10 µL NEBuffer 3
- 2 µL XbaI
- 2 µL PstI
Aliquot 10µL into each tube.
