IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-10-29: Difference between revisions
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==Midiprep of | ==Midiprep of J036335 (KaiA+KaiC), J036832 (KaiB)== | ||
Midiprep of KaiA+KaiC, KaiB was successful. | Midiprep of KaiA+KaiC, KaiB was successful. | ||
== Digest of | == Digest of J036335\ES (KaiA + KaiC), J036832\EX (KaiB) == | ||
KaiA+KaiC \ES: 6 samples | KaiA+KaiC \ES: 6 samples | ||
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* 0.2 µL Pst1 (3.4) | * 0.2 µL Pst1 (3.4) | ||
KaiB \ EX: 6 samples | |||
1. | 1. | ||
Line 20: | Line 20: | ||
* 0.2 µL Xba1 (3.4) | * 0.2 µL Xba1 (3.4) | ||
* 0.2 µL Pst1 (3.4) | * 0.2 µL Pst1 (3.4) | ||
This digest failed for some reason (EcoRI + SpeI should use NEBuffer EcoRI, not NEBuffer 2) | |||
== Digest of J036335\SP (KaiA + KaiC), J036832\XP (KaiB) == | |||
9 samples each. | |||
== Digest of | |||
== Jeff to-do == | == Jeff to-do == |
Revision as of 20:53, 29 October 2006
Midiprep of J036335 (KaiA+KaiC), J036832 (KaiB)
Midiprep of KaiA+KaiC, KaiB was successful.
Digest of J036335\ES (KaiA + KaiC), J036832\EX (KaiB)
KaiA+KaiC \ES: 6 samples
1. * 7.6 uL DNA (132.6) * 1 µL 10x BSA (17.0) * 1 µL 10x buffer #2 (17.0) * 0.2 µL Spe1 (3.4) * 0.2 µL Pst1 (3.4)
KaiB \ EX: 6 samples
1. * 7.6 uL DNA (132.6) * 1 µL 10x BSA (17.0) * 1 µL 10x buffer #2 (17.0) * 0.2 µL Xba1 (3.4) * 0.2 µL Pst1 (3.4)
This digest failed for some reason (EcoRI + SpeI should use NEBuffer EcoRI, not NEBuffer 2)
Digest of J036335\SP (KaiA + KaiC), J036832\XP (KaiB)
9 samples each.
== Digest of
Jeff to-do
At 10PM:
- CIP the A+C\S-P for 1h
- Run a gel (Ladder, undigested, digest...)