IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-10-29: Difference between revisions
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Midiprep of KaiA+KaiC, KaiB successful. | ==Midiprep of J036 (KaiA+KaiC), J036011 (KaiB)== | ||
Midiprep of KaiA+KaiC, KaiB was successful. | |||
== Digest of J036022\ES and J036011\ | |||
KaiA+KaiC \ES: 6 samples | KaiA+KaiC \ES: 6 samples | ||
Revision as of 20:51, 29 October 2006
Midiprep of J036 (KaiA+KaiC), J036011 (KaiB)
Midiprep of KaiA+KaiC, KaiB was successful.
== Digest of J036022\ES and J036011\ KaiA+KaiC \ES: 6 samples
1. * 7.6 uL DNA (132.6) * 1 µL 10x BSA (17.0) * 1 µL 10x buffer #2 (17.0) * 0.2 µL Spe1 (3.4) * 0.2 µL Pst1 (3.4)
J36010 \ EX: 6 samples
1. * 7.6 uL DNA (132.6) * 1 µL 10x BSA (17.0) * 1 µL 10x buffer #2 (17.0) * 0.2 µL Xba1 (3.4) * 0.2 µL Pst1 (3.4)
Jeff to-do
At 10PM:
- CIP the A+C\S-P for 1h
- Run a gel (Ladder, undigested, digest...)