IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-10-29: Difference between revisions
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* 0.2 µL Xba1 (3.4) | * 0.2 µL Xba1 (3.4) | ||
* 0.2 µL Pst1 (3.4) | * 0.2 µL Pst1 (3.4) | ||
== Jeff to-do == | |||
At 10PM: | |||
* CIP the A+C\S-P for 1h | |||
* Run a gel (Ladder, undigested, digest...) | |||
Revision as of 13:34, 29 October 2006
Midiprep of KaiA+KaiC, KaiB successful.
KaiA+KaiC \ES: 6 samples
1.
* 7.6 uL DNA (132.6)
* 1 µL 10x BSA (17.0)
* 1 µL 10x buffer #2 (17.0)
* 0.2 µL Spe1 (3.4)
* 0.2 µL Pst1 (3.4)
J36010 \ EX: 6 samples
1.
* 7.6 uL DNA (132.6)
* 1 µL 10x BSA (17.0)
* 1 µL 10x buffer #2 (17.0)
* 0.2 µL Xba1 (3.4)
* 0.2 µL Pst1 (3.4)
Jeff to-do
At 10PM:
- CIP the A+C\S-P for 1h
- Run a gel (Ladder, undigested, digest...)
