IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-10-29

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Line 18: Line 18:
           * 0.2 µL Xba1 (3.4)
           * 0.2 µL Xba1 (3.4)
           * 0.2 µL Pst1 (3.4)
           * 0.2 µL Pst1 (3.4)
 +
 +
== Jeff to-do ==
 +
At 10PM:
 +
* CIP the A+C\S-P for 1h
 +
* Run a gel (Ladder, undigested, digest...)

Revision as of 16:34, 29 October 2006

Midiprep of KaiA+KaiC, KaiB successful.

KaiA+KaiC \ES: 6 samples

  1.
         * 7.6 uL DNA (132.6)
         * 1 µL 10x BSA (17.0)
         * 1 µL 10x buffer #2 (17.0)
         * 0.2 µL Spe1 (3.4)
         * 0.2 µL Pst1 (3.4) 

J36010 \ EX: 6 samples

  1.
         * 7.6 uL DNA (132.6)
         * 1 µL 10x BSA (17.0)
         * 1 µL 10x buffer #2 (17.0)
         * 0.2 µL Xba1 (3.4)
         * 0.2 µL Pst1 (3.4)

Jeff to-do

At 10PM:

  • CIP the A+C\S-P for 1h
  • Run a gel (Ladder, undigested, digest...)
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