IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-10-29

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Midiprep of KaiA+KaiC, KaiB successful.
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==Midiprep of J036 (KaiA+KaiC), J036011 (KaiB)==
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Midiprep of KaiA+KaiC, KaiB was successful.
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== Digest of J036022\ES and J036011\
KaiA+KaiC \ES: 6 samples
KaiA+KaiC \ES: 6 samples

Revision as of 23:51, 29 October 2006

Midiprep of J036 (KaiA+KaiC), J036011 (KaiB)

Midiprep of KaiA+KaiC, KaiB was successful.

== Digest of J036022\ES and J036011\ KaiA+KaiC \ES: 6 samples

  1.
         * 7.6 uL DNA (132.6)
         * 1 µL 10x BSA (17.0)
         * 1 µL 10x buffer #2 (17.0)
         * 0.2 µL Spe1 (3.4)
         * 0.2 µL Pst1 (3.4) 

J36010 \ EX: 6 samples

  1.
         * 7.6 uL DNA (132.6)
         * 1 µL 10x BSA (17.0)
         * 1 µL 10x buffer #2 (17.0)
         * 0.2 µL Xba1 (3.4)
         * 0.2 µL Pst1 (3.4)

Jeff to-do

At 10PM:

  • CIP the A+C\S-P for 1h
  • Run a gel (Ladder, undigested, digest...)
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