IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-10-29

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==Midiprep of J036 (KaiA+KaiC), J036011 (KaiB)==
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==Midiprep of J036335 (KaiA+KaiC), J036832 (KaiB)==
Midiprep of KaiA+KaiC, KaiB was successful.
Midiprep of KaiA+KaiC, KaiB was successful.
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== Digest of J036022\ES and J036011\
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== Digest of J036335\ES (KaiA + KaiC), J036832\EX (KaiB) ==
KaiA+KaiC \ES: 6 samples
KaiA+KaiC \ES: 6 samples
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           * 0.2 µL Pst1 (3.4)  
           * 0.2 µL Pst1 (3.4)  
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J36010 \ EX: 6 samples
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KaiB \ EX: 6 samples
   1.
   1.
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           * 0.2 µL Xba1 (3.4)
           * 0.2 µL Xba1 (3.4)
           * 0.2 µL Pst1 (3.4)
           * 0.2 µL Pst1 (3.4)
 +
 +
This digest failed for some reason (EcoRI + SpeI should use NEBuffer EcoRI, not NEBuffer 2)
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== Digest of J036335\SP (KaiA + KaiC), J036832\XP (KaiB) ==
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9 samples each.
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 +
== Digest of
== Jeff to-do ==
== Jeff to-do ==

Revision as of 23:53, 29 October 2006

Contents

Midiprep of J036335 (KaiA+KaiC), J036832 (KaiB)

Midiprep of KaiA+KaiC, KaiB was successful.

Digest of J036335\ES (KaiA + KaiC), J036832\EX (KaiB)

KaiA+KaiC \ES: 6 samples

  1.
         * 7.6 uL DNA (132.6)
         * 1 µL 10x BSA (17.0)
         * 1 µL 10x buffer #2 (17.0)
         * 0.2 µL Spe1 (3.4)
         * 0.2 µL Pst1 (3.4) 

KaiB \ EX: 6 samples

  1.
         * 7.6 uL DNA (132.6)
         * 1 µL 10x BSA (17.0)
         * 1 µL 10x buffer #2 (17.0)
         * 0.2 µL Xba1 (3.4)
         * 0.2 µL Pst1 (3.4)

This digest failed for some reason (EcoRI + SpeI should use NEBuffer EcoRI, not NEBuffer 2)

Digest of J036335\SP (KaiA + KaiC), J036832\XP (KaiB)

9 samples each.

== Digest of

Jeff to-do

At 10PM:

  • CIP the A+C\S-P for 1h
  • Run a gel (Ladder, undigested, digest...)
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