IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-10-30

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(J36337 (A+B+C) transformants)
Line 25: Line 25:
Restreaked A-L on two LB Kan plates.
Restreaked A-L on two LB Kan plates.
-
Performed diagnostic PCR (8 uL PCR Supermix, 1 uL 2nM VF2, 1 uL 2nM VR primers) on each of the 12 colonies, along with a positive control (J36831 colony) and negative (dH2O).
+
Performed diagnostic PCR (8 uL PCR Supermix, 1 uL 2nM VF2, 1 uL 2nM VR primers) on each of the 12 colonies, along with a positive control (J36831 colony) and negative (no colony).
We also inoculated 12 liquid cultures (2 mL LB Kan+Amp), one for each colony, from the restreak plates.
We also inoculated 12 liquid cultures (2 mL LB Kan+Amp), one for each colony, from the restreak plates.

Revision as of 23:23, 30 October 2006

Contents

Making stabs

  • Heat up LB agar 10min @ 50% power in microwave
  • Aliquot out 20mL
  • Add 40uL 50mg/uL AMP
  • Aliquot 1mL into each stab

Stabs made

 BBa_J   name           frozen stock
 36801   KaiA           2006_8_13KaiA
 36802   KaiB           2006_8_13KaiB
 36804   KaiC           2006_8_13KaiC
 36831   Lac+RBS+A      A9 green tube
 36832   Lac+RBS+B      colony E 10.21
 36834   Lac+RBS+C      M2-7 8.30
 36335   LacRBSALacRBSC colony 1 10.21
 36336   LacRBSBLacRBSC J36021 10/26/06

Updated biobricks registry & sent parts

yup

J36337 (A+B+C) transformants

We have 12 colonies, labeled A-L.

Restreaked A-L on two LB Kan plates.

Performed diagnostic PCR (8 uL PCR Supermix, 1 uL 2nM VF2, 1 uL 2nM VR primers) on each of the 12 colonies, along with a positive control (J36831 colony) and negative (no colony).

We also inoculated 12 liquid cultures (2 mL LB Kan+Amp), one for each colony, from the restreak plates.

Personal tools