IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-7-13: Difference between revisions

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Tranformed Plate: One in the middle, three+ on the edge of the plate.
Tranformed Plate: One in the middle, three+ on the edge of the plate.


==Site-Specific Mutagenesis PCR==
Refer to the image for details; this is the "crossover pcr" step.
[[Image: KABC PPTZS.jpg|thumb|Refer to this for PCR overview]]
==Future Experiment Planning==
==Future Experiment Planning==
<center>'''More detailed plans for our future cyanobacteria work:'''</center>
<center>'''More detailed plans for our future cyanobacteria work:'''</center>

Revision as of 10:20, 13 July 2006



Incubator status

  • Removed
    • ...
Click here for a map of the lower shelf
(2006-07-13)

Experimental Results

The plates grown from yesterday (2nd Topo&Transformation) show the following:

Positive Control: Many colonies

Negative Control: No colonies

Tranformed Plate: One in the middle, three+ on the edge of the plate.

Site-Specific Mutagenesis PCR

Refer to the image for details; this is the "crossover pcr" step.

File:KABC PPTZS.jpg
Refer to this for PCR overview

Future Experiment Planning

More detailed plans for our future cyanobacteria work: