IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-7-16: Difference between revisions
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==Housekeeping (incubator status)== | ==Housekeeping (incubator status)== | ||
*'''Removed''' | :*'''Removed''' | ||
**All flasks from upper shelf | :**All flasks from upper shelf | ||
**PCC 7942 flask w/o thiosulfate | :***Bleach was added to the flasks and poured down the drain | ||
*'''Added''' | :**PCC 7942 flask w/o thiosulfate | ||
**Inoculated a new flask (100 mL BG-11 w/thiosulfate) with a colony from PCC 7942 streak #1 plate | :*'''Added''' | ||
**Inoculated a new flask (100 mL BG-11 w/thiosulfate) with 2 mL of liquid culture from the PCC 7942 flask w/thiosulfate (from 2006-7-7) | :**Inoculated a new flask (100 mL BG-11 w/thiosulfate) with a colony from PCC 7942 streak #1 plate | ||
**Control (100 mL BG-11 w/thiosulfate) | :**Inoculated a new flask (100 mL BG-11 w/thiosulfate) with 2 mL of liquid culture from the PCC 7942 flask w/thiosulfate (from 2006-7-7) | ||
:**Control (100 mL BG-11 w/thiosulfate) | |||
==Gel Electrophoresis== | |||
:*Ran two gels of the PCR products from Friday | |||
:**1% Agarose Gel: for larger segments | |||
:**2% Agarose Gel: for smaller segments | |||
:*The gels were hot to the touch and one of the gels crumbled (melted). | |||
:*The other gel displayed nothing (not even the ladder) | |||
:*Ran two gels again, repeat of the first gel electrophoresis | |||
:**1% Agarose Gel: for larger segments | |||
:**1.5% Agarose Gel: for smaller segments | |||
Revision as of 14:15, 16 July 2006
Housekeeping (incubator status)
- Removed
- All flasks from upper shelf
- Bleach was added to the flasks and poured down the drain
- PCC 7942 flask w/o thiosulfate
- All flasks from upper shelf
- Added
- Inoculated a new flask (100 mL BG-11 w/thiosulfate) with a colony from PCC 7942 streak #1 plate
- Inoculated a new flask (100 mL BG-11 w/thiosulfate) with 2 mL of liquid culture from the PCC 7942 flask w/thiosulfate (from 2006-7-7)
- Control (100 mL BG-11 w/thiosulfate)
- Removed
Gel Electrophoresis
- Ran two gels of the PCR products from Friday
- 1% Agarose Gel: for larger segments
- 2% Agarose Gel: for smaller segments
- The gels were hot to the touch and one of the gels crumbled (melted).
- The other gel displayed nothing (not even the ladder)
- Ran two gels again, repeat of the first gel electrophoresis
- 1% Agarose Gel: for larger segments
- 1.5% Agarose Gel: for smaller segments
- Ran two gels of the PCR products from Friday
