IGEM:Harvard/2006/Cyanobacteria/Notebook/2006-7-20: Difference between revisions
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Revision as of 15:32, 20 July 2006
To-do
- Reinoculate the Hetmann Six cultures
- Run and image gels of the colony PCR we did last night
- Send CY013 and CY014 to Genewiz again
- Transform TP (the original Topo + KaiABC plasmid) to grow up more
L; LC1 raw; LC2 raw; LC1 pure (1); LC2 pur (1); LC1 pure (10); L21 pure (10); PL1; PL2; (-); L
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L; LC1 raw; LC2 raw; LC2 pure (1); LC2 pure (10); PL1; PL2; LC1 pure (10); LC1 pure (1); (-); L
Nick's tips for gels
- When microwaving, be certain that the broth does not boil for too long, because this will evaporate the water and the % of agarose will be higher for the gel
- Take the flask out when this happens and gently swirl
- Make sure that the comb and the trays are clean
- Let the flask cool to a temperature comfortable to hold in your palm
- Pour gently to avoid bubbles
- When there are bubbles in the gel, use the larger end of a pipet tip to scoop the bubble out
- Cover the gel with foil to avoid bugs
- When microwaving, be certain that the broth does not boil for too long, because this will evaporate the water and the % of agarose will be higher for the gel
Retransformation of our first Topo + KaiABC plasmid
I retransformed our first miniprepped Topo + KaiABC plasmid since we didn't have enough plasmid to use for sequencing (Geneqiz didn't receive our order modification in time).